effect of synergistic ablation and heat shock on the rat plantaris muscle.

by Bruce Frier

Written in English
Published: Pages: 164 Downloads: 22
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The effect of synergistic ablation (SA+) and heat shock (HS+) on the rat plantaris muscle was investigated by measuring changes in HSP72, HSP25, proliferating cell nuclear antigen (PCNA), MyoD, myosin heavy chain type I (MHC I) content (mug), muscle mass (g) and protein concentration (mug/mg) over a 7-day period. Morphological changes and satellite cell expression patterns were determined histo- and immunohistochemically. The results demonstrated that HS+ prior to SA+ lead to elevated HSP72, decreased protein concentration and attenuated hypertrophy by preventing MHC I expression, whereas SA+ without HS+ results in a more gradual increase in HSP72, a similar HSP25 expression pattern as HS+ animals, reduced edema and increased MHC I protein accumulation. Satellite cell cycling was similar between HS+ and HS- plantaris muscles following SA+. It is suggested that the mechanism by which HS+ attenuates hypertrophy in the rat plantaris muscle is independent of satellite cell activation and replication.

The Physical Object
Pagination164 leaves.
Number of Pages164
ID Numbers
Open LibraryOL19747700M
ISBN 100612914941

The hindlimb soleus and plantaris muscle were functionally overloaded for 1, 3, 7, and 21 days by surgical ablation of the distal third of the lateral and medial gastrocnemius muscle as previously described (5, 28). Briefly, rats were anesthetized with an intramuscular injection of a cocktail containing ketamine hydrochloride (75 mg/kg. A sagittal-plane model of the knee, which takes account of the movements of the flexion axis relative to the femur and tibia and considers the possibility of antagonistic and synergistic muscle action, is used to determine the values of the forces transmitted by the muscles, cruciate ligaments and intra-articular surfaces during the clean phase of the clean-and-jerk weightlift. Roy, Roland R., Robert J. Talmadge, Kenneth Fox, Michael Lee, Aki Ishihara, and V. Reggie Edgerton. Modulation of MHC isoforms in functionally overloaded and exercised rat plantaris fibers.J. Appl.   Using male adult Sprague-Dawley rats, Lee et al. showed an increase in AR protein of % and % after 7 and 21 days in mechanically overloaded plantaris muscles by surgical ablation of two synergistic muscles. Co-overexpression of either SRF or active RhoA with AR indicated a synergistic and fold increase in the skeletal alpha-actin.

Cardiac ablation is a medical procedure for treating arrhythmia, irregular heartbeat, and atrial fibrillation (AFib). Learn more about the types of ablation, the procedures, possible risks and. Attenuation of age-related sarcopenia by creatine supplementation has been equivocal. In this study, plantaris muscles of young (Y; 5m) and aging (A; 24m) Fisher rats underwent four weeks of either control (C), creatine supplementation (Cr), surgical overload (O), or overload plus creatine (OCr). Creatine alone had no effect on muscle fiber cross-sectional area (CSA) or heat shock protein. Our study was designed to investigate whether creatine supplementation alone, surgical ablation alone, or a combination of creatine supplementation and surgical ablation would lead to hypertrophy in individual fiber types of the plantaris muscle. Furthermore, surgical ablation has been associated with increased expression of heat shock protein.   The regulation of skeletal muscle regeneration and growth in response to functional overload is a coordinated interaction of mechanical and endocrine signaling pathways. This study’s purpose was to determine if RhoA expression and activity in rat plantaris muscle was induced by functional overload with or without anabolic steroid administration. Male Sprague Dawley ( g) rats .

Start studying synergists and antagonists of the ankle joint and foot. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Understanding the role of muscle synergies can allow us to select functional tasks to assess different aspects of muscle function. Layers of control. Grimaldi () breaks down muscles into layers of control as follows: Deep muscle system. Primarily act to control the . Cryoballoon Ablation. Another type of ablation, known as cryoballoon ablation, uses liquid nitrogen instead of radiofrequency energy. Cryoballoon ablation precisely targets the multiple nerve connections around the opening of the pulmonary veins, which deliver oxygenated blood .

effect of synergistic ablation and heat shock on the rat plantaris muscle. by Bruce Frier Download PDF EPUB FB2

Thirty-two female rats were randomly assigned to four groups: control (C), synergistic ablation (ABL) of the gastrocnemius and plantaris muscles to overload the soleus muscle, hindlimb suspension (HLS), or a combination of synergistic ablation and hindlimb suspension (HLS-ABL).Cited by: The synergist muscle ablation model induces muscle hypertrophy in only a few days, thereby facilitating the study of adaptive responses.2,3,7,The most studied muscles are plantar flexors in the rear paw of by:   Although the soleus muscle comprises only 6% of the ankle plantar flexor mass in the rat, a major role in stance and walking has been ascribed to it.

Cited by: 7. Plantaris muscle hypertrophy was induced by a unilateral ablation of synergistic muscles after a week. Food intake of soy protein-fed mice was higher than that of caseinate and whey-fed mice.

OR “ablation of synergists” AND “compensatory hyper - trophy” AND “experimental models” OR “skeletal muscle cross-sectional area.” The following criteria were used for the selection of papers: (1) the use of a rat model; (2) the use of synergist ablation to overload the plantaris muscle.

Skeletal muscle can undergo rapid growth in response to a sudden increase in work load. For example, the rat soleus muscle increases in weight by 40% within six days after the tendon of the.

MG muscle neurovascular grafting and synergist muscle ablation procedures. As previously described by Larkin et al. (), the tendon and nerve of the left MG muscle were severed, and the muscle was grafted orthotopically with repair of the nerve and y, animals were anesthetized with pentobarbital sodium (65 mg/kg).

Mechanical loading can increase tendon cross-sectional area (CSA), but the mechanisms by which this occurs are largely unknown. To gain a greater understanding of the cellular mechanisms of adult tendon growth in response to mechanical loading, we used a synergist ablation model whereby a tenectomy of the Achilles tendon was performed to induce growth of the synergist plantaris tendon.

Early response of heat shock proteins to functional overload of the soleus and plantaris in rats and mice Effects of gender and functional overload on plantaris muscle morphology in the dwarf (HsdOla:dw-4) Lewis rat.

Life Sciences, Vol. 65, No. 23 Synergistic ablation does not affect atrophy or altered myosin heavy chain expression in. muscle ablation. To study the effect of nerve-repair grafting on met-abolic and contractile function in skeletal muscle, we needed to find a muscle that is large enough to collect adequate samples for determining several assays of metabolic function and that has an accessible muscle with a fiber-type composition comparable to that of humans.

Synergist ablation surgery involves the surgical removal of the gastrocnemius and soleus muscles resulting in functional overload of the remaining plantaris muscle. This functional overload results in myofiber hypertrophy, as well as the activation, proliferation, and. Start studying Muscles: Action, Antagonist and Synergist.

Learn vocabulary, terms, and more with flashcards, games, and other study tools. The purpose of this study was to investigate whether the soleus muscle undergoes atrophy and alterations in myosin heavy chain (MHC) composition durin.

rat plantaris muscle. It was hypothesized that elevating Hsp content within the plantaris muscle via a single heat stress, prior to subjecting the plantaris muscle to overload by surgical removal of synergistic muscles, would ame-liorate any acute stress effects and thus lead to greater hypertrophy.

MATERIALS AND METHODS. The choice between a centrifugal radial approach and a centripetal convergent one depends on several clinical factors ().When several small targets need to be treated simultaneously (paucimultinodular disease) and/or at close time intervals (recurrent multicentric forms of disease), the simplicity and speed of conventional centrifugal ablative techniques emerge as essential benefits.

Rat PLT muscle was subjected to compensatory overload following removal of the gastrocnemius and soleus of each hindlimb. Animals were randomly assigned to control and overload groups. Overload muscles were harvested at 3 days and 1, 2, and 4 wk after synergistic ablation.

The actual ablation process is quite simple and involves the delivery of approximately cc of local anesthetic through the probe cannula, just prior to ablation, normally at 90ºC for 90 seconds.

The tip of the probe creates a sphere of heat sufficient to disrupt the myelin sheath, which is. Start studying Prime Mover, Synergists, Antagonists, Fixators. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Start studying Lower Leg Muscles - Action, Antagonist, Synergist. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Although the “heat sink” effect of tumor perfusion is a primary contributor to ablation outcome (10, 11), multiple other factors—including electrical and thermal conductivity—have been observed to influence coagulation in ex vivo tissues, suggesting further underlying differences in the sensitivity of tissues to RF energy application.

A work-induced hypertrophy model, employing the removal of the synergists involved in plantar flexion (synergistic ablation) of the rat hindlimb, is well established; previous studies have documented the biochemical and anthropomorphic changes to the rat plantaris muscle following ablation-induced overload (Noble et alLocke et al Effects of heat shock transcription factor 1 (HSF1) gene on the regrowth of atrophied mouse soleus muscles were studied.

Protein synthesis in rat plantaris muscle undergoing surgically induced. Synergists (down) and Antagonists (Across) Glenohumerol Joint (Ball and Socket): Flexion: Extension: Deltoid (Anterior Fibers). The changes induced by overload model in plantaris muscle of rats resulted in a significant increase muscle mass (up to an increase of 10% to 30% in the cross section of the muscle fibers seconds.

The time course of angiogenesis during hypertrophy of the rat plantaris muscle was studied by using a unilateral, synergistic ablation model. Animals (n = 6/group) were euthanized 2, 5, 7, 15, 21, and 30 days ns from both the hypertrophied and contralateral muscles were simultaneously stained for capillaries and muscle fiber type.

Heat shock protein 20 was the least responsive, increasing only in 7 day FO rat plantaris compared with control rats. Overall, the results demonstrate that levels of both large and small HSPs increase with FO, suggesting a contributory role during the compensatory hypertrophy response.

Heat shock proteins (Hsps) are molecular chaperones that aid in protein synthesis and trafficking and have been shown to protect cells/tissues from various protein damaging stressors. To determine the extent to which a single heat stress and the concurrent accumulation of Hsps influences the early events of skeletal muscle hypertrophy, Sprague-Dawley rats were heat stressed (42°C, 15.

The purpose of this study was to determine if heat shock could provide protection to skeletal muscle as has been shown in cardiac muscle. A minute heat shock at 42 degrees C resulted in an. Synergistic Co-activation Increases the Extent of Mechanical Interaction between Rat Ankle Plantar-Flexors The Harvard community has made this article openly available.

Please share how this access benefits you. Your story matters Citation Tijs, Chris, Jaap. In the rat hindlimb, muscle fibre types differ widely between soleus (86% type I) and the muscles making up the white portion of the gastrocnemius (95% type IIB), with varying proportions in other sections of the gastrocnemius–plantaris–soleus (GPS) complex (Delp & Duan, ).

Surgical ablation is an animal model involving surgical removal of a muscle, thereby overloading its synergistic muscles and Heat shock protein for plantaris muscle in young and aging animals for Age-related alterations in expression of apoptosis regulatory proteins and heat shock proteins in rat skeletal muscle.

Biochimica et. The diversity of muscle fibers allows them to perform specialized functional tasks. Thus the type I fibers with high oxidative capacity and capillary density are more suitable for endurance exercise while type IIb fibers with low oxidative capacity and capillary density are suitable for short term IIa are the intermediate fiber types that allow high power generation at a considerable.These results indicate that effects of LG+PL MTU length changes on F SO were enhanced by synergistic muscle activation.

ANOVA indicated no effect of LG+PL length on the HRT of F SO (Figure (Figure3), 3), both during SO excitation (average across LG+PL lengths: ± ms, p = ) and during SO&LG excitation (average across LG+PL.